Composite

Part:BBa_K1725430:Design

Designed by: Andrey Filipov   Group: iGEM15_Glasgow   (2015-09-08)


J23101 + RBS + UirR + B0015 + PlsiR + GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 206
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1389
    Illegal BsaI.rc site found at 2137
    Illegal SapI.rc site found at 510


Design Notes

J23101, UirR, and B0015 were ligated to Plsir and GFP after restriction that was followed by an Azure-A-based gel extraction.


Source

UirR and Plsir come from the genomic sequence of the unicellular cyanobacterium Synechocystis sp. PCC6803.

References